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. 2018 Aug 13;9:217. doi: 10.1186/s13287-018-0958-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Differentiation of CBMC-iPSC-Fs. a Schematic of fibroblast differentiation process. b Morphology of iPSC-Fs on day 30. Scale bars, 100 μm. c Gene expression of pluripotency marker OCT4 and fibroblast markers CD44, COL1A1, COL1A2, COL3A1, and vimentin. d Immunocytochemical analysis of fibroblast markers fibronectin (red) and vimentin (red), together with DAPI staining (blue). Scale bars, 100 μm. e Flow cytometric analysis of CD34-positive, CD45-positive, CD73-positive, and CD105-positive cells. Graphs show mean with SEM of five independent samples. **p < 0.01, ***p < 0.001. EB embryoid body, FDM fibroblast differentiation medium, GAPDH glyceraldehyde 3-phosphate dehydrogenase, iPSC induced pluripotent stem cell, iPSC-F iPSC-derived fibroblast, n.s. not significant