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. 2018 Aug 13;18:114. doi: 10.1186/s12935-018-0612-1

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — a TargetScan, miRDB and RNA22 are used to search for potential target genes of miR-524. After down-regulation of miR-524 expression in MG-63 (b) and 143B (c) cell lines, mRNA expression levels of each candidate target gene is analysed by qRT-PCR. d After down-regulation of miR-524 in MG-63 and 143B, PTEN protein expression was significantly increased. e miR-524 and PTEN 3′UTR binding sequences and PTEN 3′UTR mutant sequences. f, g Luciferase reporter assay used to verify direct binding between miR-524 and PTEN (***P < 0.001, **P < 0.01, *P < 0.05)