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. 2018 Aug 13;15:228. doi: 10.1186/s12974-018-1252-z

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Maternal VAC and MIA effects on cortical lamination in the developing brain at E18.5. a Coronal sections of embryonic brains were immunostained with Tbr1 (green), Satb2 (red), and Hoechst (blue). Tbr1 mainly indicates the cells in cortical layers V–VI, while Satb2 labels layer II–V cells. Scale bar, 100 μm. b MIA induced a significant reduction of Satb2⁺ cells (p = 0.015), and VAC preprocessing reversed the effect (p = 0.025). c A similar but not significant trend was observed for Tbr1⁺ cells in the cortical plate (p > 0.05). n = 6 mice/group; *p < 0.05 (two-way ANOVA and Student’s t test). The results are all shown as the mean + s.e.m.