Fig. 1.

Separation of 37-nt and 58-nt fragments. TapeStation traces of an input mix of two single-stranded DNA oligonucleotides, 37 nt and 58 nt, separated by double-sided size selection on SPRI beads. The first size-selection step was performed at three different isopropanol conditions, 38% (a, b, c), 41% (d, e, f), and 44% (g, h, i). Input oligonucleotide mixtures for each concentration are presented in (a, d, g). Eluates of the first size-selection step using each isopropanol concentration are presented in (b, e, h). Eluates of the second size selection using each isopropanol concentration are presented in (c, f, i). Peak sizes and corresponding fragments areas are marked in blue; the left peak titled “Lower” is a 25-nt size marker