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. 2018 Aug 13;37:189. doi: 10.1186/s13046-018-0855-7

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effect of MKRN2 in the presence or absence of PI3K inhibition on protein expression in NSCLC cells. a A549 and b H1299 cells transfected with si-MKRN2 were treated or not treated with the PI3K inhibitor LY294002 analyzed by western blot for the expression of proteins involved in cell migration and invasion. Relative quantification analysis was based on grayscale values. *P < 0.05; **P < 0.01. c Interactions between MKRN2 and PI3Kp85α in A549 and H1299 cells as measured by co-immunoprecipitation. d The relationship between MKRN2 expression and p-Akt (Ser473) levels as analyzed by immunohistochemistry