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. 2018 Aug 13;37:189. doi: 10.1186/s13046-018-0855-7

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — MKRN2 mediates PI3Kp85α ubiquitination and degradation to affect the Akt pathway. a, b mRNA levels of the p85α subunit of PI3K in a A549 and b H1299 cells transfected with si-MKRN2. c Relative PI3Kp85α protein expression (normalized to GAPDH) as measured by densitometry. *P < 0.05; **P < 0.01. d, e Effects of MKRN2 expression on PI3Kp85α ubiquitination. d A549 and e H1299 cells transfected with si-MKRN2 or the MKRN2-expression plasmid along with HA-ubiquitin (Ub). Levels of PI3Kp85α ubiquitination were evaluated by immunoprecipitation using the anti-PI3Kp85α antibody, followed by anti-HA immunoblotting