Fig. 5.

HDAC4 expression in pBECs from non-asthmatics and asthmatics cultured at ALI. Cells were infected with IAV H1N1 (MOI 5) and HDAC4 expression was assessed at different time points. a and (b) represent mRNA expressions of HDAC4 at baseline and after IAV H1N1 infection (6–24 h) in non-asthmatics and asthmatics, respectively. The cycle threshold (Ct) value was normalized to 18S gene (∆Ct) and data are presented relative to corresponding non-asthmatics at baseline. *P ≤ 0.05 intra-cohort, and #P ≤ 0.05 inter-cohort between corresponding time points, using the Kruskal Wallis multiple comparisons test and Mann-Whitney test, respectively, N=5. c and (d) are immunoblots representative of baseline and 24 h after infection in non-asthmatics and asthmatics, respectively. e and (f) represent densitometric quantification of immunoblots. Data are presented relative to corresponding non-asthmatic levels at baseline. *P ≤ 0.05, using Mann-Whitney test, N=4