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. 2018 Aug 2;19:146. doi: 10.1186/s12931-018-0849-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — a Total HA accumulation as assessed by enzyme linked sorbent assay (ELSA) was greater in HLFs co-cultured with asthmatic BECs compared to HLFs co-cultured with healthy BECs (P = 0.04, N = 7 /group). b Quantitative radiolabeling of HA demonstrated that HLFs co-cultured with asthmatic BECs produced greater total amounts of HA compared to HLFs co-cultured with healthy BECs (P < 0.02, N = 7 /group). c HLFs co-cultured with asthmatic BECs demonstrated a greater abundance of HMW-HA and intermediate weight HA species compared to HLFs co-cultured with healthy BECs. A representative plot of HA-sensitive material as measured by S-1000 size exclusion chromatography is shown. Data is plotted as disintegrations per minute (DPM) vs. the partition coefficient (K_av)