Fig. 5.

Adhesion of U937 cells to ECM generated by HLFs following 96 h of co-culture with either healthy or asthmatic BECs. Representative images of U937 cell adhesion obtained by fluorescent microscopy depicting binding patterns observed for a HLFs co-cultured with healthy BECs and b HLFs co-cultured with asthmatic BECs. Pre-treatment with hyaluronidase decreased U937 binding to HLFs co-cultured with either c healthy BECs or d asthmatic BECs. Quantitative analysis of U937 cell binding using a plate-based assay revealed greater adhesion of U937 cells by HLFs co-cultured with asthmatic BECs compared to healthy BECs (P < 0.0001; E). Data shown as mean ± SD for N = 10 replicates/group