Fig. 8.

CAPS marker analysis of T₁ plants germinated from seeds of control sweet orange (C1–1 to C10–3) and transgenic sweet orange (T1 to T10). DNA isolated from wild type sweet orange leaves (C-L) and transgenic sweet orange leaves (T-L) was used as a reference template. T₁ plants with an underline had different genotypes from the control sweet orange and mother tree. Identical genotypes to sweet orange for the three CAPS markers means the T₁ plant germinated from a polyembryo, while a different genotype means the T₁ plant germinated from a zygotic embryo produced by self-pollination