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. 2018 Aug 13;19:605. doi: 10.1186/s12864-018-4974-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Validation of DEGs and DELs in dry and lactation periods by qRT-PCR. Relative expression of DEGs (FGFBP1, IGFBP5, LPO, CXCL10, and SAA3) and DELs (TCONS_00040268, TCONS_00137654, TCONS_00071659, TCONS_00000352, TCONS_01118313, TCONS_ 00093001 and NONBTAG0105046.2) were verified by qRT-PCR, and the results indicated that the relative expressions of TCONS_00040268, TCONS_00137654, TCONS_00071659, TCONS_00000352 and TCONS_ 00093001 in lactation were significantly higher than that in dry stage (P < 0.05). On the contrary, CXCL10 and TCONS_01118313 in lactation were lower than that in dry stage (P < 0.05). The qRT-PCR results were consistent with the sequencing data