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. 2018 Aug 7;10:58. doi: 10.1186/s13073-018-0570-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Effect of CAD knockdown on Junín virus minigenome replication and transcription. To exclude an unspecific role of CAD on T7-driven minigenome systems in general, Junín virus (JUNV) minigenomes encoding nano-luciferase (nLuc) were replicated and transcribed in cells pretransfected with siRNAs against CAD, NXF1, or control siRNAs (no siRNA; neg. siRNA: negative control siRNA; killer siRNA: cytotoxic siRNA as transfection control). As an additional control the JUNV polymerase was omitted from the assay (no L). In parallel, an ATP-based cell viability assay was performed using UltraGlo luciferase activity as a readout. Means from 3 different experiments are shown