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. 2018 Aug 13;16:223. doi: 10.1186/s12967-018-1592-6

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Analysis of exosomes by flow cytometry using CD63 coated magnetic beads. Exosomes were first visualized on forward (FSC) versus sidescatter (SSC) plots to gate on the respective exosomes bound to beads population, after gating on singlets. a Typical SSC versus PE-CD63 plots for exosomes isolated from the serum of two donor samples. As comparison, a typical plot for serum derived exosomes from prostate cancer patients using CD63 antibodies at baseline and post-radiation. b Typical SSC versus FITC-A plots for exosomes using CD9 antibodies at baseline and post-radiation. The graph shows increased CD63 representing exosomes compared to CD9 representing surface markers after radiotherapy