Fig. 8.

Blocking endocytosis and autophagy modulates the internalization and degradation of aSyn. a ICC of Rab4A-GFP expressed in H4 cells treated with 1 μM aSyn monomers and with vehicle, PitStop 30 μM or with Dyngo 50 μM. Both PitStop and Dyngo are inhibitors of the endocytic processes. b Quantification of the aSyn mean fluorescence intensity in the three conditions. c ICC of Rab7-GFP-expressing cells treated with 1 μM aSyn monomers and with vehicle, Bafilomycin 100 nM (Baf 100 nM), or with Chloroquine 50 μM (Chlq 50 μM). Bafilomycin and Chloroquine are inhibitors of lysosomal acidification and, therefore, autophagy-lysosome pathway (ALP). (D) Quantification of the aSyn mean fluorescence intensity in the three conditions. Scale bar: 30 μm