Table 1.
List of the blackfly species, country of collection, number of specimens with DNA barcodes (n) and mean intraspecific values of sequence divergence (%)
| Species | Collection country | n | Mean sequence divergence (%) |
|---|---|---|---|
| Prosimulium hirtipes | Spain, UK | 11 | 0.62 |
| Prosimulium latimucro (s.l.)a | Spain, UK | 5 | 2.93 |
| Prosimulium rufipes (s.l.) | Spain | 4 | 0.82 |
| Prosimulium tomosvaryi a | Spain | 17 | 2.77 |
| Simulium (Boophthora) erythrocephalum | Spain | 13 | 0.42 |
| Simulium (Eusimulium) angustipes | UK | 9 | 1.09 |
| Simulium (Eusimulium) petricolum | UK | 5 | 1.66 |
| Simulium (Eusimulium) rubzovianumb | Spain, UK | 16 | 1.63 |
| Simulium (Nevermannia) bertrandi | Spain | 10 | nac |
| Simulium (Nevermannia) brevidens | Spain | 7 | 0.05 |
| Simulium (Nevermannia) carthusiense | Spain | 10 | 0.35 |
| Simulium (Nevermannia) cryophilum (s.l.) | Spain | 7 | 0.81 |
| Simulium (Nevermannia) vernum (s.l.) | Spain, UK | 11 | 0.71 |
| Simulium (Simulium) intermediuma | Spain | 21 | 3.96 |
| Simulium (Simulium) ornatum (s.l.) | Spain | 13 | 1.62 |
| Simulium (Simulium) argyreatuma | Spain, UK | 17 | 2.71 |
| Simulium (Simulium) monticola | Spain | 14 | 0.45 |
| Simulium (Simulium) variegatuma | Spain, UK | 22 | 2.15 |
| Simulium (Simulium) xanthinum | Spain | 1 | nac |
| Simulium (Trichodagmia) galloprovinciale | Spain | 2 | 0.17 |
| Simulium (Wilhelmia) equinum | Spain | 5 | 0.56 |
| Simulium (Wilhelmia) lineatum | Spain | 8 | 1.11 |
| Simulium (Wilhelmia) pseudequinum | Spain, UK | 6 | 1.19 |
| Simulium (Wilhelmia) sergenti | Spain | 15 | 0.35 |
aTaxa with high level of genetic diversity
bThe name of S. rubzovianum replaced S. velutinum for previous distributional records of this species in Spain, which is now restricted to North Africa and Canary Islands [43]
cMean intraspecific values of sequence divergence (K2P) shown with missing entries (na) indicate that less than two specimens were analyzed