Fig. 5.

Expression of homologous genes shared between C. psittaci and C. abortus. a The normalized expression (DESeq2) of 34 virulence and immunogenic factors present in C. psittaci and C. abortus but absent in W. chondrophila are shown. Significance threshold is depicted by asterisk (*) and represents Benjamini-Hochberg adjusted p-value < 0.01 and an absolute log2-fold change > 1.0. Homologous virulence factors for which no gene name was found are specified with the C. psittaci NCBI locus tag. b Confirmation of the RNA-seq results with RT-qPCR for five genes shared among C. psittaci and C. abortus. The Pearson correlation coefficient (> 0.97) demonstrates the high degree of correlation between the RT-qPCR and RNA-seq results in both the intra- and inter-species comparison of gene expression. Standard deviation of log2-fold changes are indicated