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. 2018 Aug 2;13:27. doi: 10.1186/s40248-018-0137-4

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Panel A. Immunohistochemistry staining for INK4 locus. A. INK4 locus immunohistochemistry staining of sample 4, group +/−. B. INK4 locus immunohistochemistry staining of sample 3, group ++. C. INK4 locus immunohistochemistry staining of sample 47, group +++. Panel B. Immuno-staining groups and relative percentages (40 + 90 cases analyzed). Panel C. Sensitivity and quantitative accuracy of MTAP SMART-MSP assay (90 cases analyzed). Top: The assay was sensitive to 0,1% methylation in a background of WGA. Bottom: The assay was quantitative accurate in the range 100% methylation to 0,1% methylation (R2 = 0,99,393). The PCR efficiency was 0,95