Fig. 3.

Correlation of baseline cfDNA concentration quartiles with rPFS and OS. (A) Kaplan-Meier curve of rPFS by baseline log₁₀ cfDNA concentration quartiles. (B) Forest plot for rPFS (multivariable analysis model) for baseline log₁₀ cfDNA concentration for each study and combined estimate. (C) Kaplan-Meier curve of OS by baseline cfDNA concentration quartiles. (D) Forest plot for OS (multivariable analysis model) for baseline log₁₀ cfDNA concentration for each study and combined estimate. The multivariable Cox model included baseline log₁₀ cfDNA concentration, ECOG PS at baseline (0 vs 1–2), visceral metastases, bone-only disease, Gleason score, baseline pain, baseline albumin, baseline ALP, baseline haemoglobin, baseline LDH, baseline NLR and baseline PSA. The I² test displays and tests the level of heterogeneity between the studies, which is nonsignificant for cfDNA. ALP = alkaline phosphatase; cfDNA = cell-free DNA; CI = confidence interval; ECOG PS = Eastern Cooperative Oncology Group performance status; HR, = hazard ratio; LDH = lactate dehydrogenase; NLR = Neutrophil-lymphocyte ratio; OS = overall survival; PSA = prostate-specific antigen; rPFS = radiological progression-free survival.