Table 1.
Combination index values of AZD-1775 in combination with clofarabine
| 24 h | 48 h | 72 h | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Clofarabine (nM) | Clofarabine (nM) | Clofarabine (nM) | |||||||||
| Cell lines | 2.5 | 5 | 10 | 2.5 | 5 | 10 | 2.5 | 5 | 10 | ||
| REH | AZD-1775 (nM) | 6.9 | 0.70 | 0.80 | 0.95 | 0.61 | 0.88 | 0.99 | 0.78 | 1.07 | 1.12 |
| 20.6 | 0.80 | 0.70 | 0.94 | 0.85 | 0.95 | 1.06 | 0.78 | 1.02 | 1.15 | ||
| 61.7 | 1.32 | 0.70 | 0.91 | 1.61 | 1.38 | 1.05 | 0.90 | 1.10 | 1.09 | ||
| 185.2 | 1.64 | 0.70 | 0.82 | 2.56 | 2.02 | 0.92 | 1.26 | 1.09 | 0.81 | ||
| 555.6 | 0.89 | 0.46 | 0.65 | 1.00 | 0.82 | 0.55 | 0.43 | 0.24 | 0.29 | ||
| 1666.7 | 1.10 | 0.43 | 0.76 | 0.33 | 0.34 | 0.34 | 0.62 | 0.64 | 0.68 | ||
| 5000.0 | 2.23 | 1.11 | 0.77 | 1.00 | 0.26 | 1.01 | 1.81 | 1.83 | 1.88 | ||
| NALM-6 | AZD-1775 (nM) | 6.9 | 0.24 | 0.45 | 0.55 | 0.60 | 0.46 | 0.75 | 0.73 | 1.06 | 1.15 |
| 20.6 | 0.20 | 0.30 | 0.49 | 0.49 | 0.56 | 0.96 | 0.76 | 1.02 | 1.17 | ||
| 61.7 | 0.26 | 0.22 | 0.39 | 0.66 | 0.76 | 0.93 | 0.84 | 1.08 | 1.14 | ||
| 185.2 | 0.45 | 0.34 | 0.33 | 1.99 | 1.31 | 0.80 | 1.24 | 1.16 | 0.97 | ||
| 555.6 | 0.63 | 0.39 | 0.27 | 1.38 | 0.93 | 0.66 | 0.85 | 0.66 | 0.57 | ||
| 1666.7 | 0.55 | 0.36 | 0.33 | 9.87 | 0.84 | 1.04 | 0.20 | 0.26 | 0.37 | ||
| 5000.0 | 0.84 | 0.64 | 0.71 | 2.05 | 1.98 | 2.19 | 0.49 | 0.55 | 0.66 | ||
| NALM-19 | AZD-1775 (nM) | 6.9 | 0.46 | 0.54 | 0.85 | 0.52 | 0.59 | 0.81 | 1.02 | 1.20 | 1.09 |
| 20.6 | 0.45 | 0.43 | 0.60 | 0.35 | 0.54 | 0.68 | 1.00 | 1.18 | 1.08 | ||
| 61.7 | 0.56 | 0.39 | 0.62 | 0.38 | 0.41 | 0.43 | 0.91 | 1.01 | 0.92 | ||
| 185.2 | 0.73 | 0.32 | 0.44 | 0.49 | 0.35 | 0.20 | 0.91 | 0.79 | 0.73 | ||
| 555.6 | 0.60 | 0.21 | 0.32 | 0.20 | 0.14 | 0.18 | 0.53 | 0.50 | 0.74 | ||
| 1666.7 | 0.68 | 0.27 | 0.41 | 0.21 | 0.15 | 0.27 | 0.69 | 0.75 | 1.10 | ||
| 5000.0 | 1.39 | 0.76 | 0.69 | 0.56 | 0.53 | 0.70 | 1.75 | 1.79 | 2.43 | ||
| RPMI-8402 | AZD-1775 (nM) | 6.9 | 0.21 | 0.26 | 0.50 | 0.23 | 0.36 | 1.39 | 8.20 | 1.23 | 1.46 |
| 20.6 | 0.14 | 0.24 | 0.63 | 0.34 | 0.42 | 11.91 | 0.69 | 0.78 | 1.43 | ||
| 61.7 | 0.22 | 0.21 | 0.62 | 0.77 | 0.53 | 3.17 | 0.55 | 0.83 | 1.26 | ||
| 185.2 | 0.41 | 0.26 | 0.81 | 1.48 | 1.43 | 2.37 | 0.66 | 0.80 | 0.73 | ||
| 555.6 | 0.57 | 0.36 | 0.54 | 0.95 | 0.61 | 0.48 | 0.43 | 0.41 | 0.08 | ||
| 1666.7 | 0.60 | 0.38 | 0.32 | 0.55 | 0.33 | 0.21 | 0.28 | 0.10 | 0.12 | ||
| 5000.0 | 0.37 | 0.26 | 0.05 | 0.09 | 0.02 | 0.04 | 0.22 | 0.24 | 0.26 | ||
| Cell lines | 5 | 10 | 20 | 5 | 10 | 20 | 5 | 10 | 20 | ||
| CCRF-CEM | AZD-1775 (nM) | 6.9 | 0.01 | 0.02 | 0.23 | 0.74 | 1.13 | 1.15 | 1.01 | 1.61 | 1.06 |
| 20.6 | 0.01 | 0.01 | 0.08 | 0.92 | 2.29 | 1.30 | 0.43 | 2.41 | 1.02 | ||
| 61.7 | 0.01 | 0.01 | 0.02 | 0.64 | 1.07 | 0.70 | 4.20 | 4.81 | 0.98 | ||
| 185.2 | 0.01 | 0.01 | 0.02 | 1.13 | 0.78 | 0.29 | 0.91 | 0.83 | 0.76 | ||
| 555.6 | 0.01 | 0.01 | 0.01 | 0.20 | 0.19 | 0.22 | 0.39 | 0.51 | 0.72 | ||
| 1666.7 | 0.05 | 0.06 | 0.09 | 0.44 | 0.42 | 0.45 | 0.80 | 0.94 | 1.08 | ||
| 5000.0 | 0.42 | 0.45 | 0.30 | 1.40 | 1.22 | 0.96 | 2.02 | 2.11 | 1.87 | ||
| MOLT-4 | AZD-1775 (nM) | 6.9 | 0.00 | 0.09 | 0.25 | 1.62 | 8.99 | 8.15 | 1.00 | 1.03 | 1.27 |
| 20.6 | 0.11 | 0.15 | 0.32 | 2.26 | 0.85 | 10.07 | 0.80 | 1.14 | 1.02 | ||
| 61.7 | 0.32 | 0.24 | 0.40 | 0.69 | 0.75 | 0.55 | 0.95 | 1.19 | 0.62 | ||
| 185.2 | 0.57 | 0.27 | 0.23 | 1.24 | 0.94 | 0.43 | 0.81 | 0.53 | 0.26 | ||
| 555.6 | 0.31 | 0.16 | 0.18 | 0.88 | 0.61 | 0.40 | 0.00 | 0.11 | 0.12 | ||
| 1666.7 | 0.40 | 0.28 | 0.30 | 1.31 | 1.42 | 0.42 | 0.31 | 0.31 | 0.32 | ||
| 5000.0 | 1.61 | 1.76 | 1.66 | 6.06 | 5.49 | 5.24 | 0.92 | 2.04 | 0.93 | ||
The table reports the Combination Index (CI) value for each combination of drugs. CI < 1 indicates synergism, CI = 1 indicates additivity and CI > 1 indicates antagonism