Table 3.
Combination index values of AZD-1775 in combination with bosutinib isomer (Bos-I) on BCR-ABL1-negative cell lines
| 24 h | 48 h | 72 h | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Bosutinib isomer (nM) | Bosutinib isomer (nM) | Bosutinib isomer (nM) | |||||||||
| Cell line | 500 | 1000 | 2000 | 500 | 1000 | 2000 | 500 | 1000 | 2000 | ||
| NALM-6 | AZD-1775 (nM) | 6.9 | 0.57 | 0.69 | 0.79 | 0.62 | 1.00 | 1.21 | 0.59 | 0.79 | 1.14 |
| 20.6 | 0.33 | 0.48 | 0.64 | 0.65 | 0.81 | 1.07 | 0.47 | 0.68 | 0.99 | ||
| 61.7 | 0.35 | 0.39 | 0.65 | 0.67 | 0.65 | 0.97 | 0.68 | 0.73 | 0.93 | ||
| 185.2 | 0.48 | 0.41 | 0.41 | 0.91 | 0.65 | 0.68 | 0.89 | 0.73 | 0.63 | ||
| 555.6 | 0.63 | 0.43 | 0.36 | 0.54 | 0.45 | 0.47 | 0.41 | 0.40 | 0.48 | ||
| 1666.7 | 0.67 | 0.51 | 0.41 | 0.65 | 0.66 | 0.68 | 0.53 | 0.59 | 0.66 | ||
| 5000.0 | 1.65 | 1.43 | 1.21 | 1.58 | 1.61 | 1.62 | 1.42 | 1.43 | 1.52 | ||
| Cell lines | 250 | 500 | 1000 | 250 | 500 | 1000 | 250 | 500 | 1000 | ||
| NALM-19 | AZD-1775 (nM) | 6.9 | 0.43 | 0.75 | 1.01 | 1.99 | 2.94 | 0.20 | 0.19 | 0.28 | 0.35 |
| 20.6 | 0.38 | 0.47 | 0.63 | 1.53 | 1.15 | 0.12 | 0.24 | 0.29 | 0.28 | ||
| 61.7 | 0.19 | 0.27 | 0.23 | 0.20 | 0.12 | 0.09 | 0.44 | 0.48 | 0.31 | ||
| 185.2 | 0.27 | 0.24 | 0.24 | 0.42 | 0.23 | 0.15 | 0.67 | 0.48 | 0.26 | ||
| 555.6 | 0.38 | 0.31 | 0.28 | 0.41 | 0.31 | 0.25 | 0.29 | 0.28 | 0.21 | ||
| 1666.7 | 0.76 | 0.68 | 0.69 | 0.82 | 0.72 | 0.69 | 0.47 | 0.44 | 0.38 | ||
| 5000.0 | 1.78 | 1.95 | 1.77 | 2.04 | 2.09 | 1.93 | 1.19 | 1.30 | 1.13 | ||
| MOLT-4 | AZD-1775 (nM) | 6.9 | 1.23 | 0.93 | 1.11 | 0.99 | 1.03 | 1.28 | 0.67 | 1.22 | 1.26 |
| 20.6 | 0.78 | 0.61 | 0.78 | 0.85 | 0.89 | 1.07 | 0.74 | 0.89 | 1.07 | ||
| 61.7 | 0.77 | 0.53 | 0.43 | 0.62 | 0.63 | 0.71 | 0.91 | 0.85 | 0.77 | ||
| 185.2 | 0.53 | 0.40 | 0.23 | 0.39 | 0.35 | 0.34 | 0.35 | 0.37 | 0.53 | ||
| 555.6 | 0.22 | 0.25 | 0.21 | 0.34 | 0.40 | 0.50 | 0.35 | 0.43 | 0.67 | ||
| 1666.7 | 0.40 | 0.60 | 0.65 | 0.95 | 1.12 | 1.14 | 0.92 | 0.96 | 1.17 | ||
| 5000.0 | 2.46 | 2.25 | 3.02 | 3.06 | 3.26 | 3.49 | 3.54 | 3.30 | 3.52 | ||
| Cell line | 2000 | 5000 | 10000 | 2000 | 5000 | 10000 | 2000 | 5000 | 10000 | ||
| CCRF-CEM | AZD-1775 (nM) | 6.9 | 0.75 | 0.78 | 0.84 | 0.75 | 0.78 | 0.84 | 1.06 | 0.65 | 0.39 |
| 20.6 | 0.38 | 0.62 | 0.79 | 0.38 | 0.62 | 0.79 | 1.21 | 0.57 | 0.52 | ||
| 61.7 | 0.27 | 0.54 | 0.74 | 0.27 | 0.54 | 0.74 | 0.53 | 0.49 | 0.52 | ||
| 185.2 | 0.26 | 0.46 | 0.58 | 0.26 | 0.46 | 0.58 | 0.28 | 0.43 | 0.49 | ||
| 555.6 | 0.25 | 0.65 | 0.53 | 0.25 | 0.65 | 0.53 | 0.35 | 0.49 | 0.37 | ||
| 1666.7 | 0.84 | 0.93 | 0.81 | 0.84 | 0.93 | 0.81 | 0.66 | 0.79 | 0.46 | ||
| 5000.0 | 3.13 | 2.76 | 0.99 | 3.13 | 2.76 | 0.99 | 2.08 | 1.97 | 0.88 | ||
The table reports the Combination Index (CI) value for each combination of drugs. CI < 1 indicates synergism, CI = 1 indicates additivity and CI > 1 indicates antagonism