Fig. 1.

Cross-species comparison of the liver proteome integrated with RNA-seq from the same animals. a Liver proteomes from four adult naked mole-rats (NMRs) and four guinea pigs (GPs) were compared using quantitative mass spectrometry. Hierarchical clustering based on the correlation between proteome profiles based on 3248 protein groups quantified across the two species. b Comparison of NMR/GP fold changes estimated at the protein level by quantitative mass spectrometry and at the transcript level by RNA-seq. Colored dots indicate genes significant (q < 0.1) in both comparisons. The names of selected genes that show consistent fold changes in the two comparisons are indicated. For RNA-seq analysis, only reads mapping to conserved regions between the two species were considered. c Heatmap showing NFE2L2 target genes significantly affected in NMR vs. GP. List of NFE2L2 basal target genes was obtained from [30]. Only genes significant at both the transcript and protein level (q < 0.1, corresponding to a combined q < 0.05) are shown. Related to Additional file 1: Table S1, Additional file 2: Table S2, and Additional file 6: Figure S2