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. 2018 Aug 7;8:301. doi: 10.3389/fonc.2018.00301

Figure 2.

Figure 2

Effect of APRIL and BAFF on migration and EMT status. (A) Effect of APRIL and BAFF (100 ng/ml) on migration of T47D and MDA cells after 24–48 h of treatment. Figure presents mean ± SE of three independent experiments. (All samples were analyzed with t-test comparing at each time point, treatment vs. control, and *denotes p < 0.05) (B). Representative photos (Magnification 1000x) of control (untreated) T47D cells and APRIL or BAFF (100 ng/ml) treated cells for 4 days immunostained in suspension for keratins (upper panel, green) and vimentin (lower panel, red). Fluorescence intensity was calculated using Image J. Values represent the ratio of vimentin/keratins expression in these cells. (C) Graphical presentation of EMT status changes obtained by calculating the ratio of vimentin/keratins expression of 50 cells for each treatment condition. All samples were analyzed with t-test (*** denotes p < 0.0001).