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. 2018 Aug 7;8:301. doi: 10.3389/fonc.2018.00301

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Copyright © 2018 Pelekanou, Notas, Athanasouli, Alexakis, Kiagiadaki, Peroulis, Kalyvianaki, Kampouri, Polioudaki, Theodoropoulos, Tsapis, Castanas and Kampa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression levels of pluripotency markers in T47D cells after APRIL or BAFF treatment. (A) Expression levels of ALDH1A1, KLF4, SOX-2, and c-MYC, genes (quantified by Real-Time PCR and expressed as percentage of control) after treatment of T47D cells with APRIL or BAFF (100 ng/ml) for 4 days. Three independent experiments were performed (mean ± SE, *denotes p < 0.05, at least). (B,C) Expression of SOX-2, c-MYC, ALDH1A1, and NANOG proteins (assayed by immunocytochemistry). Representative images of three independent experiments are presented (B) Quantification of the proteins' expression levels was performed in at least 10 cells in three different photos for both control and treated cells using Image J and is expressed as percentage of control (mean ± SE, (C)).