Skip to main content
. 2018 Aug 7;8:301. doi: 10.3389/fonc.2018.00301

Figure 5.

Figure 5

(A) Effect of APRIL and BAFF on breast cancer stem cells in the presence of siBCMA. Percentage increase in the number of breast cancer stem cells transfected or untransfected with siBCMA and treated with BAFF or APRIL (100 ng/ml) for 4 days. (Breast cancer stem cell number was estimated by counting the cells with high green autofluoresence in a BL2-A/BL1-A dot plot). (B) NFk-B activity of untreated (control) and treated with APRIL or BAFF (100 ng/ml, for 24h) T47D cells. Experiments were performed in triplicate. (C,D) Effect of APRIL and BAFF on ALDH1A1 and KLF4 expression in the presence of a JNK inhibitor or sh RNA for JNK1 or JNK2. Expression levels of ALDH1A1 and KLF4 after treatment of T47D cells with APRIL or BAFF (100 ng/ml) for 4 days in the presence of a JNK inhibitor SP600125 (10 μM) (C) or sh RNA for JNK1 or JNK2 (D). Data are presented as a mean ± SE of three independent experiments (*denotes p < 0.05, at least).