Fig. 5.

RIF1 regulated hTERT expression and promoter activity in EOC cells by binding to the promoter of hTERT. a and b hTERT mRNA expression was down-regulated in RIF1 knockdown and up-regulated in RIF1 overexpressed OVCAR3 and A2780 cells. c RIF1 regulated the protein expression of hTERT and the hTERT downstream PI3K/AKT signaling proteins in OVCAR3 and A2780 cells. d Relative hTERT promoter activity in RIF1 knockdown and RIF1 overexpressed OVCAR3 and A2780 cells. e Schematic diagram of five pairs of primers designed for ChIP spanning the hTERT promoter. f RIF1 binds to the hTERT promoter indicated by ChIP in OVCAR3 and A2780 cells. IgG from rabbit served as a control. g A schematic diagram of RIF1 wild type and mutant binding sites in hTERT promoter. h Dual-luciferase reporter gene assay was conducted in OVCAR3 and A2780 cells with overexpression of RIF1. Data are expressed as means ± SD of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001