Fig. 1.
Validation of GPR120 knockout mice. a Agarose gels demonstrate Neor, Gpr120, and Gapdh amplification products in mouse genomic DNA. b The GPR120 mRNA level was detected by real-time PCR in colon (positive control tissue) of wild-type (WT) and homozygous knockout (Homo KO) mice (n = 4/group). c Agarose gels demonstrate Gapdh and Gpr120 amplification products in mouse colon cDNA. d The presence of β-galactosidase, a surrogate for GPR120 in the KO mouse, is detected by immunofluorescence in mouse colon tissue. Colon sections from WT (upper) and homo KO (bottom) mice were stained with antibody against beta-galactosidase (red). Magnification of the image ×100. e The body weight of the two group do not show statistical difference