Fig. 5.

The GPR120 agonist DHA exhibits anti-inflammatory effects on primary human chondrocytes. a Agarose gels demonstrate Gapdh and Gpr120 amplification products in human colorectal tumor cell Caco2 (positive control) and human chondrocyte cell cDNA. b Human chondrocytes were exposed to 50 ng/ml human tumor necrosis factor alpha (TNFα) with or without 25 μM docosahexaenoic acid (DHA) for 48 h. The mRNA expression levels of the proinflammatory genes Ccl2, Cox2, IL-1β, and MMP13 were assessed (n = 6/group). *p < 0.05, **p < 0.01, ****p < 0.0001 compared with corresponding vehicle group. NS not significant