Fig. 2.

ScGrx6 holoprotein stabilization in aerobic solutions by the addition of DTT, EDTA, GSH, GSSG or H₂O₂ at 2 mM. The absorbance of freshly purified ScGrx6 at 430 nm was measured at six different time points in the absence and presence of DTT, EDTA, GSH, GSSG or H₂O₂. The absorbance of the buffer containing 20 mM sodium chloride and 50 mM tris hydrochloride, pH 8.0, was subtracted using a reference cuvette. All assays were performed at 25 °C. The concentration of ScGrx6 in the assays was 2 μM. Time points: 0, 50, 100, 150, 200 and 250 min; blank is free of all additives. The experiment was repeated three times