Fig. 6.

MiR-301b regulates the inhibitory effect of ISL on melanoma by functionally targeting LRIG1. a 7 common target genes predicted by TargetScan and GEO database were selected as potential target genes of miR-301b. b The western blot analysis of LRIG1 protein level in A375 and A2058 cells after being treated with miR-301b minics/inhibitor. c The effect of miR-NC, miR-301b on luciferase (luc) activity in A375 cells transfected with either the LRIG1–3’UTR WT or the LRIG1–3’UTR MUT. *P < 0.05 vs miR-NC group, n = 3. d Immunohistochemical analysis of LRIG1 in tumor sections of PBS + angomir-ctr group, ISL + angomir-ctr group and ISL + miR-301b angomir group. e The RT-qPCR analysis of LRIG1 in tumor samples of PBS + angomir-ctr group, ISL + angomir-ctr group and ISL+ miR-301b angomir group. f The western blot analysis of apoptosis associated proteins(LRIG1, c-PARP, Bax, bcl-2, C-caspase-3) in ISL treated A375 and A2058 cells which were transfected with LRIG1 siRNA or scramble vector in advance. g The flow cytometry analysis of apoptosis in ISL treated A375 and A2058 cells which were transfected with LRIG1 siRNA or scramble vector in advance. h, i Representative images of TUNEL staining in ISL treated A375 and A2058 cells which were transfected with LRIG1 siRNA or scramble vector in advance. *P < 0.05, **P < 0.01 vs PBS + angomir-ctr group, n = 3