The body core temperature of mice was raised to the indicated levels by a thermal feedback circuit and heat lamp in steps of 0.5°C per two minutes, and seizures were recorded by video camera and implanted EEG electrodes. (a) Representative EEG traces during a spontaneous seizure in a DS mouse. (b) Representative EEG traces of F/+:Dlx-Cre- and F/+:Dlx-Cre+ mice during thermal induction of seizures at P24. Top, F/+:Dlx-Cre- mouse at 39.5°C. Bottom, F/+:Dlx-Cre+ mouse during a seizure at 39.5°C. (c) Temperature dependence of thermal induction of seizures in DS mice. Thermally-induced seizures were evoked in DS mice at P30–46 (circles, mean temperature of 39°C) and P20–22 (squares, mean temperature of induction 40.2°C), but no seizures were induced in P17–18 mice (triangles). (d) Temperature dependence of thermal induction of seizures in Dlx-Cre+ mice. Thermally-induced seizures were evoked in all F/+:Dlx-Cre+ animals with a mean temperature of 39°C. No F/+:Dlx-Cre- mice had a seizure.