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. 2018 Jul 31;2018:7468019. doi: 10.1155/2018/7468019

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Copyright © 2018 Wenliang Zha et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Curcumin decreased DM-induced apoptosis in the testis. (a) Representative images of apoptotic cells stained by TUNEL (magnification=400×; bar indicates 20 μm). (b) Representative immunohistochemical staining of Bcl-2 (magnification=200×; bar indicates 50 μm). (c) Representative immunohistochemical staining of Bax (magnification=200×; bar indicates 50 μm). (d) Representative images of Bcl-2 and Bax protein; β-actin served as the loading control. (e) Quantitative analysis of Bcl-2 expression. (f) Quantitative analysis of Bax expression. (g) Analysis of TUNEL-positive cells. n=4. Values are presented as the mean±SD. ^∗P<0.05 versus the control group. ^#P<0.05 versus the DM group.