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. 2018 Jul 31;2018:3817057. doi: 10.1155/2018/3817057

Figure 4.

Figure 4

miR-1907 activates hepatocyte autophagy by inhibiting GSK3 β. CCL-9.1 cell proliferation was assayed after miR-1907 treatment with or without 3-MA (1 μM). (b) Quantification of PCNA-positive cells in mice liver injected with miR-1907 with or without 3-MA (2mg/kg) at different timepoints before and after 2/3 PH. (c) Mice were injected with miR-1907 with or without 3-MA before and after 2/3 PH. The liver mass to body weight ratio was then calculated at different timepoint. (d) Schematic representation of the miR-1907 site in GSK3β 3′-UTR. (e) The 3′UTR reporter assay was carried out in CCL-9.1 cells overexpressed with miR-1907. pGL3-GSK3β-3′-UTR-WT or pGL3-GSK3β-3′-UTR-Mutation was cotransfected with pRL-TK. Luciferase assays were performed 48 h after transfection. Firefly luciferase activity was standardized to Renilla luciferase control. (f) Western blot analysis for endogenous GSK3β protein level after miR-1907 overexpression or inhibition in CCL-9.1 cells. (g) CCL-9.1 cells were treated with miR-1907 with or without 3-MA, and the autophagosome formation was visualized by assaying activated green puncta. Scale bar, 50 μm. ∗p < 0.05.