Figure 2.

NKA drives Ca²⁺responses in both neurons and enteric glia. (A) Representative Ca²⁺ responses evoked by NKA in enteric neurons and glia. Gray traces show the responses of individual glial cells and blue traces show the responses of individual neurons. (B and C) Representative Ca²⁺ responses evoked by NKA in a single enteric neuron (blue traces) and the surrounding enteric glia (gray traces) showing examples of a (B) neuron response that proceeds glial responses and a (C) neuronal response that follows glial responses. (D) Number of responses and (E) peak response per neuron or glia when challenged with NKA (n = 7 cells from 2 mice; Student t test). (F) Temporal-code images of enteric glial responses to adenosine diphosphate (ADP) or NKA. Images are Z-projections of time series images from peak responses of enteric glia to ADP or NKA where cells that respond first are shown in blue (1 s after drug application) and cells that respond later are shown in red (30 s after drug application). Note that ADP responding cells respond faster, and at the same time compared with NKA responding cells. Scale bar: 50 μm. (G) Representative glial Ca²⁺ responses evoked by either ADP (dashed gray traces, bottom) or NKA (solid green traces, top).