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. 2018 May 29;6(3):321–344. doi: 10.1016/j.jcmgh.2018.05.009

Figure 3.

Figure 3

NK1R expression is localized to enteric neuron cell bodies and varicosities whereas NK2R is expressed primarily by neuronal varicosities surrounding enteric glia. Images (confocal single optical planes, 1 μm) show triple-label immunohistochemistry for GFAP (green, A-D), Hu (blue, A’–D’), and either NK1R (magenta, A’’-B’’) or NK2R (magenta, C’’–D’’) in the distal colon myenteric plexus of mice. Preadsorption control is shown for NK1R (B–B’’’’) and NK2R (D–D’’’’). Images show triple-label immunohistochemistry for GFAP (green, E), tdTomato-tagged TRPV1-positive varicosities (blue, E’), and NK2R (magenta, E’’) in the distal colon myenteric plexus of mice. (A’’’–E’’’) Overlays of each combination. Areas demarked by the dashed boxes in A’’’–E’’’ are enlarged in panels A’’’’–E’’’’. (E’’’) Scale bar: 20 μm (applies to A–E’’’). Scale bars, 10 μm in the enlarged images (A’’’’, B’’’’, C’’’’, D’’’’, and E’’’’). Labeling is representative of experiments performed on a minimum of 3 mice.