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. 2018 May 29;6(3):321–344. doi: 10.1016/j.jcmgh.2018.05.009

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

NKA drives Ca²⁺responses in TRPV1-positive neuronal varicosities. (A) Schematic of transgene containing the tdTomato-tagged genetically encoded Ca²⁺ indicator GCaMP5g driven by the TRPV1 promoter. (B) Confocal image of dual-label immunohistochemistry (single optical plane, 1 μm) for the tdTomato-tagged GCaMP5g (magenta) and enteric glia (GFAP, green) in the myenteric plexus of the TRPV1^tm1(cre)Bbm/J::Polr2a^{tm1(CAG-GCaMP5g,-tdTomato)Tvrd} mouse distal colon. Scale bar: 20 μm. (C–C’) Representative traces of average Ca²⁺ responses from TRPV1-positive neuronal fiber varicosities in response to (C) 1 μmol/L capsaicin (CAP) or (C’) 1 μmol/L NKA. Recordings were obtained from 3 to 5 tissue preparations from 3 mice.