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. 2018 May 29;6(3):321–344. doi: 10.1016/j.jcmgh.2018.05.009

Figure 7.

Figure 7

The expression of tachykinins and their receptors is not altered during inflammation. (A) Representative single optical section (1 μm) images of triple-label immunohistochemistry for the pan-neuronal marker Hu (blue), NKA (green), and SP (magenta) in saline and DNBS mice treated with vehicle or GR 159897 at 48 hours or 3 weeks after induction of colitis. Scale bar: 30 μm (applies to all images). Labeling is representative of experiments performed on a minimum of 3 mice. Quantification of (B) NKA and (C) SP integrated density in saline and DNBS mice treated with vehicle or GR 159897 at 48 hours or 3 weeks after induction of colitis (n = 3–4 mice; 2-way analysis of variance with multiple comparisons, normalized to group vehicle saline). Quantification of (D) TACR1 and (E) TACR2 mRNA expression from the distal colons of healthy or inflamed mice treated with vehicle or GR 159897 at 48 hours or 3 weeks after induction of colitis (n = 3–6 mice; 2-way analysis of variance with multiple comparisons; normalized to glyceraldehyde-3-phosphate dehydrogenase [GAPDH]; *P = .0344).