Figure 6.
YAP1 was negatively regulated by miR-497.
Notes: (A) qRT-PCR detection of YAP1 mRNA expression in each group; (B) Western blot to detect YAP1 protein expression in each group; (C) immunofluorescence detection of YAP1-positive cells proportion in each group; (D) TargetScan prediction of the binding site for YAP1 and miR-497; (E) dual luciferase reporter gene activity assay. *P<0.05 when compared with NC group or WT+NC group.
Abbreviations: MT, mutant type; NC, negative control; WT, wild type.