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. Author manuscript; available in PMC: 2018 Aug 14.
Published in final edited form as: Cell Rep. 2018 Jun 26;23(13):3776–3786. doi: 10.1016/j.celrep.2018.05.078

Figure 4. Let-7 Has Developmental and Adult-Specific Roles.

Figure 4.

(A and B) Deletions of the entire let-7 complex (let-7C), most of the let-7.5p sequence (let-7Δ13−43), or the let-7.5p seed sequence (let-7Δ12−15), assayed as heterozygotes (gray plots) or trans-heterozygotes (orange plots), decreased day sleep in LD and DD.

(A)Sleep per 30 min (top) and total day and night sleep (bottom) in let-7 mutants in LD. let-7 trans-heterozygotes had reduced day sleep (Kruskal-Wallis test, p < 0.05).

(B)Sleep per 30 min (top) and total sleep (bottom) in let-7 mutants in DD. All trans-heterozygous let-7 mutants had reduced sleep (Kruskal-Wallis test, p < 0.05).

(C)Let-7SP was restricted to the larval and pupal stages by shifting tubGal4, tubGal80ts > let-7SP flies from 29°C to 18°C after eclosion. Day sleep was significantly decreased (Mann-Whitney test, p < 0.05).

(D)Let-7SP expression was restricted to adulthood by shifting tubGal4, tubGal80ts > let-7SP from 18°C to 29°C after eclosion. Night sleep was significantly decreased (Mann-Whitney test, p < 0.05).

Error bars represent SEM; whiskers represent maxima and minima. Numbers are reported next to the genotype. See also Figures S2 and S3.