REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-Mtln	custom Proteintech	N/A
Mouse CI monoclonal antibody (20E8C12)	ThermoFisher	Cat# A21348; RRID:AB_2535839
Mouse VDAC1 monoclonal antibody (N152B/23)	NeuroMab	Cat# 75–204; RRID: AB_2214807
Rabbit anti-subunit c of the mitochondrial ATP synthase Antibody	a gift from Dr. Elizabeth F. Neufeld	N/A
Total OXPHOS Rodent WB Antibody Cocktail	Abcam	Cat# ab110413, RRID:AB_2629281
Rabbit polyclonal OGDH antibody	ABclonal	Cat# A6391
Rabbit polyclonal ACAD9 antibody	AVIVA	Cat# OAAN02440
Rabbit Anti-VLCAD Polyclonal Antibody	GeneTex	Cat# GTX114232S, RRID:AB_10728154
Rabbit Anti-SOD2 Polyclonal Antibody	a gift from Dr. Frederick E. Domann	N/A
Rabbit Anti-Glutathione Peroxidase 1 Polyclonal Antibody	Abcam	Cat# ab22604, RRID:AB_2112120
Rabbit monoclonal Glutathione Peroxidase 4 antibody	Abcam	Cat# ab125066, RRID:AB_10973901
Rabbit anti-Beta-catenin Antibody	Sigma-Aldrich	Cat# PLA0230
Peroxidase-AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu,Bov,Hrs,Rb,Sw Sr Prot) antibody	Jackson ImmunoResearch Labs	Cat# 115–035–146, RRID:AB_2307392
Peroxidase-AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu,Ms,Rat Sr Prot) antibody	Jackson ImmunoResearch Labs	Cat# 111–035–144, RRID:AB_2307391
Mouse Anti-NDUFA9 Monoclonal Antibody, Clone 20C11	Abcam	Cat# ab14713, RRID:AB_301431
Rabbit Anti-Human LETM1 Polyclonal Antibody	GeneTex	Cat# GTX112455, RRID:AB_1950806
Rabbit polyclonal CYCS (Cytochrome C) antibody	ABclonal	Cat# A0225
Rabbit Anti-GAPDH Polyclonal antibody	Santa Cruz Biotechnology	Cat# sc-25778, RRID:AB_10167668
Pyruvate Dehydrogenase (C54G1) Rabbit mAb antibody	Cell Signaling Technology	Cat# 3205S, RRID:AB_2162926
Rabbit polyclonal DLD antibody	ABclonal	Cat# A13296
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568	Thermo Fisher Scientific	Cat# A-11011, RRID:AB_143157
Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488	Thermo Fisher Scientific	Cat# A32723, RRID:AB_2633275
Mouse Anti-FLAG Monoclonal Antibody, Clone M2	Sigma-Aldrich	F3165 RRID:AB_259529
Mouse Anti-UQCRC1 Monoclonal Antibody, Clone 16D10AD9AH5	Abcam	Cat# ab110252 RRID: AB_10863633
Chemicals, Peptides, and Recombinant Proteins
Human Mtln (uniprot Q8NCU8–2) 56 amino acid protein, 96% purity by HPLC	Genescript	Custom order
MitoTracker® Red CMXRos	Invitrogen	M7512
MitoSOX Red Mitochondrial Superoxide Indicator	Invitrogen	M36008
Critical Commercial Assays
Worthington Neonatal Cardiomyocyte Isolation System	Worthington Biochemical Corporation	LK003300
Cardiolipin Beads	Echelon Biosciences	P-BCLP
Control Beads	Echelon Biosciences	P-B000
Lipid Strips	Echelon Biosciences	P-6002
Glutathione Assay Kit	Cayman Chemicals	Item 703002
EnzyChrom Aconitase Assay Kit	BioAssay Systems	EACO-100
Cardiolipin Assay Kit, Fluorometric	BioVision	K944–100
Experimental Models: Cell Lines
C2C12 myoblast	ATCC	CRL-1772
HeLa	ATCC	CCL-2
Neuro-2a (N2a)	ATCC	CCL-131
Experimental Models: Organisms/Strains
Mouse: B6SJLF1/J	The Jackson Laboratory	JAX: 100012
Mouse: C57BL/6J		JAX: 000664
Mouse: Mtln knockout mice backcrossed to C57BL/6J	This paper	N/A
Oligonucleotides
DNA oligonucleotides	Integrated DNA Technologies (IDT)	See Table S4
Custom Dicer-substrate siRNAs targeting human Mtln	IDT	See Table S4
Scrambled Dicer-substrate siRNA	IDT	IDT #1074346
Negative Control Dicer-substrate siRNA	IDT	IDT #1074354
Recombinant DNA
Multiplex Genome Engineering Using CRISPR/Cas Systems.		Addgene Plasmid #42229
Multiplex Genome Engineering Using CRISPR/Cas Systems.		Addgene Plasmid #42230
Ad5CMVcytoLacZ (Ad-ßgal)	University of Iowa Viral Vector Core	VVC-U of Iowa-3554
Ad5TRENC116 (Ad-Mtln))	University of Iowa Viral Vector Core	(Iowa-custom, Ad5TREnc116)
Ad5CMVeGFP (Ad-GFP)	University of Iowa Viral Vector Core	VVC-U of Iowa-4
Software and Algorithms
coxpresdb ver.6	NA	http://coxpresdb.jp/
ToppFun gene ontology enrichment tool; ToppGene Suite	NA	https://toppgene.cchmc.org/
RONN v3.2	NA	https://www.strubi.ox.ac.uk/RONN
IUPred	NA	iupred.enzim.hu/
PrDOS	NA	prdos.hgc.jp/cgi-bin/top.cgi