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. Author manuscript; available in PMC: 2018 Aug 14.
Published in final edited form as: J Control Release. 2017 Apr 5;255:36–44. doi: 10.1016/j.jconrel.2017.03.397

Figure 3. Reactogenicity at the site of vaccine inoculation.

Figure 3.

A. Photos were taken in 1, 3, 5 and 10 days after inoculation. Only areas of inflammatory and abnormal skins were outlined by a white-dot line in all panels. B. Cross-sections of the inoculation site were H&E stained 3 days after inoculation and scanned by Nanozoomer; a red circle indicates an area of inflammation induced by BCG in the skin and arrows point the sites of a hypodermic needle or microneedle insertion. Scale bar, 200 μm. C. Differences of skin surface temperature between the inoculation site and a distant area were recorded daily for 10 days (n=6). Statistical significance was analyzed by ANOVA, *P<0.05,**P<0.01, and ***P<0.001 in the presence or absence of BCG.