Fig. 3.

Co-silencing NaPDR1-like and NaPDR1 lead to plants highly susceptible to A. alternata. a) Mean (±SE) NaPDR1 transcripts were measured by real-time PCR in six replicates of 0 leaves of EV and VIGS PDR1 and 1-like plants at 1 dpi and 3 dpi. b) Mean (±SE) NaPDR1-like transcripts were measured by Real-time PCR in six replicates of 0 leaves of EV and VIGS PDR1 and 1-like plants at 1 dpi and 3 dpi. c) Photographs were taken of two 0 leaves of EV plants and two 0 leaves of VIGS PDR1 and 1-like plants at 6 dpi. White arrows indicate lesions. d) Mean (±SE) diameter of necrotic lesions of fifteen replicates of 0 leaves of EV, VIGS PDR1 and 1-like plants at 6 dpi. Asterisks indicate the level of significant differences between EV and VIGS PDR1 and 1-like plants (Student's t-test: *, P < 0.05; **, P < 0.01; ***, P < 0.005).