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. 2018 Aug 2;14(8):e1007229. doi: 10.1371/journal.ppat.1007229

Fig 9. Neutrophil-mediated killing requires germination.

Fig 9

A. Macrophage-deficient (irf8-/-) or control (irf8+/+) larvae were injected with live or heat-killed TFYL49.1 (CEA10), fixed 1 dpi, and stained for neutrophils (sudan black). Larvae were then scored for the presence or absence of a sudan black cluster or mass. This experiment was performed twice, data are shown from one representative replicate. B. Wild-type or phagocyte-deficient (pu.1 morphant) larvae were infected with TBK1.1 (Af293 pyrG+), Af293.1 (Af293 pyrG-), TFYL49.1 (CEA10 pyrG+), or CEA17 (CEA10 pyrG-) spores and survival was monitored. Data represent 2 pooled experiments. Average injection CFUs: Af293 pyrG+ = 52, Af293 pyrG- = 52, CEA10 pyrG+ = 38, CEA10 pyrG- = 48. C. Wild-type larvae were infected with TBK1.1 (Af293 pyrG+), Af293.1 (Af293 pyrG-), TFYL49.1 (CEA10 pyrG+), or CEA17 (CEA10 pyrG-) and CFUs were monitored. Average injection CFUs: Af293 pyrG+ = 45, Af293 pyrG- = 48, CEA10 pyrG+ = 42, CEA10 pyrG- = 49. D. Macrophage-deficient (irf8-/-) larvae were infected with CEA17 KU80Δ (CEA10 pyrG+), or CEA17 (CEA10 pyrG-) and CFUs were monitored. Average injection CFUs: CEA10 pyrG+ = 41, CEA10 pyrG- = 45. All CFU data represent lsmeans ± SEM from 3 pooled replicates composed of 24 larvae (8 larvae per replicate) per condition per day. P values were calculated by ANOVA. See also S9 Fig.