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. 2018 Aug 2;12(8):e0006691. doi: 10.1371/journal.pntd.0006691

Fig 1. Splenomegaly and infection kinetics of the male reproductive tract during acute infection with Asian genotype ZIKV strains.

Fig 1

Mice were inoculated s.c. with 3 log10 PFU of ZIKV strains PRVABC59 (blue), P6-740 (yellow), and FSS13025 (maroon) (n = 12 per virus strain). At 6, 8, 10 and 12 days post-inoculation (dpi), three mice from each group were euthanized. (A) Viral titers in the testis. (B) Viral titers in the epididymis. The dashed line represents the limit of detection for the plaque assay. (C) Viral titers in the seminal vesicles. (D) Spleen weights were recorded and the ratio of (spleen to body weight) x 100 was measured. (E) Testicular weight as a percentage of total body weight. (F) Spermatozoa concentration decreased during acute infection. Epididymides were minced in warm PBS and a portion of the resulting cell suspension was stained with trypan blue and counted on a hemocytometer. Each symbol represents one mouse. P-values were determined by 2way ANOVA. *, p<0.05, ** p<0.01, ***p < 0.0001.