Fig 5. Testicular leukocytes contained genomic ZIKV RNA.
Mice were inoculated s.c. with 3 log10 PFU of ZIKV strains PRVABC59 (blue symbols), P6-740 (yellow), and FSS13025 (maroon) (n = 12 per virus strain). At six, eight, ten and twelve dpi, three mice from each group were euthanized. A single cell suspension of each testis was stained according to the PrimeFlow assay. (A) Representative dot plots showing testicular cells from uninfected control mice and ZIKV-infected mice stained with anti-CD45 antibody. (B) Percentage of ZIKV RNA (+) strand cells. Uninfected control mice were used to set gates for ZIKV RNA+ cells (gates were set such that 0.2% or less of the total population in the uninfected control was found in the ZIKV RNA+ gate.). (C) CD45+ leukocyte infiltration into the testes over time. (D) Percentage of ZIKV RNA (+) strand leukocytes within ZIKV RNA (+) strand cells. Each symbol represents one mouse. P-values were determined by 2way ANOVA. *, p<0.05, *** p<0.0001.