Skip to main content
. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: J Immunol. 2018 May 16;201(1):183–192. doi: 10.4049/jimmunol.1701569

Figure 7. Lys371, Lys420, and Lys500 of MAVS are necessary for TRIM29-mediated signaling.

Figure 7

(A) Immunoblot analysis of Myc-tagged MAVS and its mutations (top blot), HA-tagged TRIM29 (middle blot) and β-actin (bottom blot) in HEK293T cells cotransfected with an expression vector for HA-tagged TRIM29 and expression vectors for Myc-tagged wild-type full-length MAVS and its mutations. (B) Immunoblot analysis of HA-tagged TRIM29 (top blot), Myc-tagged MAVS and its mutations (second blot), β-actin (third blot) and K11-linked ubiquitination (bottom blot) in HEK293T cells cotransfected with expression vector for Myc-tagged MAVS or its mutations (above lanes) and expression vector for HA-tagged TRIM29, with treatment of 5 μM MG132 (above lanes). (C,D) Activation of the IFN-β promoter (C) or NF-κB promoter (D) in human HEK293T cells transfected with an IFN-β promoter luciferase reporter (C) or NF-κB promoter luciferase reporter (D), plus expression vector (each 100 ng) for wild-type MAVS or various MAVS mutants alone or expression vector for TRIM29 plus wild-type MAVS or MAVS mutants; results are presented relative to those of renilla luciferase (cotransfected as an internal control). Data are representative of three independent experiments with similar results (mean + s.d.)