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. 2018 Jul 20;7:e35783. doi: 10.7554/eLife.35783

Figure 4. Integrin regulates actin structures at a distance, through vinculin.

(A) F-actin distribution (white) in wild type and vinculin- IFMs at the stages indicated. Yellow asterisks and yellow arrow indicate regions of disorganized actin. (B) Muscle defects do not result in reduced flying ability in the vinculin mutant. 30 flies per genotype were tested and percentage that fly is shown. (C) F-actin distribution (white) in IFMs of the genotype indicated. Vinculin tail and VBS2 were expressed with mef2-Gal4. White arrows indicate zebra bodies (abnormally large Z-lines). Yellow indicate detaching myofibrils. (D) TEM images of MTZs at the indicated stage and genotype. Orange arrows indicate electron dense aggregates in the MTZ. Yellow arrowheads indicate loss of electron dense material from the muscle, but not tendon cell, membrane.

Figure 4.

Figure 4—figure supplement 1. Defects seen with loss of vinculin are rescued by adding back wild type vinculin.

Figure 4—figure supplement 1.

(A) F-actin distribution in IFMs of the genotypes indicated. The vinculin mutant phenotype is rescued by vinculin expressed either from its own promoter, p{vinculin}, or overexpressed with mef2-Gal4, mef2 >vinculin. Overexpression of vinculin alone induces some zebra bodies (white arrow). (B) Quantitation of the distance from myofibril end to first M-line. Note log scale.