Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Sarcophilus harrisii) |
Saha-UK | Murchison et al. (2012) | ensembl: ENSSHAG00000002942 |
Devil_ref v7.0 |
| Gene (S. harrisii) | Saha-UC | Murchison et al. (2012) | ensembl: ENSSHAG00000000117 |
Devil_ref v7.0 |
| Gene (S. harrisii) | Saha-UD | Murchison et al. (2012) | ensembl: ENSSHAG00000010776 |
Devil_ref v7.0 |
| Cell line (S. harrisii) | DFT1_4906 |
Siddle et al. (2013) and Murchison et al. (2012) |
RRID:CVCL_LB78; DFTD 4906; 86T |
Devil Facial Tumour 1; Supplementary file 1 |
| Cell line (S. harrisii) | DFT2_RV | Pye et al. (2016b) | DFT2_202T1 | Devil Facial Tumour 2; Supplementary file 1 |
| Cell line (S. harrisii) | DFT2_SN | Pye et al. (2016b) | DFT2_203T3 | Devil Facial Tumour 2; Supplementary file 1 |
| Cell line (S. harrisii) | DFT2_549 | This paper | Devil Facial Tumour 2; Supplementary file 1 |
|
| Cell line (S. harrisii) (Female) |
Fibroblasts_Salem | Murchison et al. (2012) | 91 H | Tasmanian devil fibroblasts |
| Cell line (Cricetulus griseus) |
CHO_SahaIFNy | Siddle et al. (2013) | Chinese Hamster Ovary (CHO) cell line transfected with pcDNA3_SahaIFNy |
|
| Biological sample (S. harrisii) |
DFT2_RV | Pye et al. (2016b) | DFT2_202T1 | Devil Facial Tumour 2; Supplementary file 1 |
| Biological sample (S. harrisii) |
DFT2_SN | Pye et al. (2016b) | DFT2_203T2 | Devil Facial Tumour 2; Supplementary file 1 |
| Biological sample (S. harrisii) |
DFT2_818 | Stammnitz et al. (2018) | Devil Facial Tumour 2; Supplementary file 1 |
|
| Biological sample (S. harrisii) |
DFT2_547 | Stammnitz et al. (2018) | 807T1 | Devil Facial Tumour 2; Supplementary file 1 |
| Biological sample (S. harrisii) |
DFT2_523 | Stammnitz et al. (2018) | 638T1 | Devil Facial Tumour 2; Supplementary file 1 |
| Biological sample (S. harrisii) |
DFT2_812 | Stammnitz et al. (2018) | Devil Facial Tumour 2; Supplementary file 1 |
|
| Biological sample (S. harrisii) |
TD_RV | Stammnitz et al. (2018) | 202H1 | Tasmanian devil spleen biopsy |
| Biological sample (S. harrisii) |
TD_SN | Stammnitz et al. (2018) | 203 H | Tasmanian devil kidney biopsy |
| Biological sample (S. harrisii) |
TD_818 | Stammnitz et al. (2018) | 818 | Tasmanian deil spleen biopsy |
| Antibody | α-UA/UB/UC_15-25-18; Classical MHC class I Saha-UA, -UB and -UC |
This paper | UA/UB/UC_15-25-18 | Antibody recognising MHC class I molecules,SahaUA, UB and UC. Generated using a peptide immunogen (WMEKVQDVDPGYWE). Supernatant from hybridoma used neat. |
| Antibody | α-UK_15-29-1; Non-classical MHC class I Saha-UK |
This paper | α-UK_15-29-1 | Antibody recognising MHC class I molecule,Saha-UK. Generated using a peptide immunogen (RITHRTHPDGKVTL). Supernatant from hybridoma used neat. |
| Antibody | IgG1 Isotype control | Sigma Aldrich | clone: MOPC-21; cat number: M5284 |
0.5 mg/ml |
| Antibody | IgG2b Isotype control | Sigma Aldrich | clone: MOPC-141; cat number: M5534 |
0.5 mg/ml |
| Antibody | a-B2m | Siddle et al. (2013) | SahaB2m-13-34-48 | supernatant used neat; B2-microglogulin |
| Antibody | a-CD3 | Dako/Agilent | cat number: A0452 | 1:50 |
| Recombinant DNA reagent |
pET22B+-SahaUC | This paper | SahaI*UC (SahaI*27) amplified using primer Saha349 and Saha350 (Supplementary file 2). |
|
| Recombinant DNA reagent |
pET22B+-SahaUK | This paper | Saha-UK in Pet22B + using primer Saha335 and Saha351 (Supplementary file 2). |