Gene expression levels for SP (TAC1, A) and CGRP (CALCA, B and CALCB, C) were determined in the lumbar DRGs by real time PCR and SP (D) and CGRP (E) protein levels in the sciatic nerve were determined by EIA. No changes in DRG SP and CGRP gene expression or sciatic nerve protein expression were observed in the fracture limb at 7 weeks post fracture (FX+No Wheel), compared to nonfractured control mice (Control), and 4 weeks of wheel running in fracture mice had no effect (FX+Wheel). Values are means ± SD, n=8 per cohort. One-way analysis of variance with Bonferroni post hoc testing.