SK-N-DZ neuroblastoma cells were inoculated subcutaneously to 20 nude
mice (1 tumor per mouse). Tumors were size matched and mice were randomized into
4 treatment groups: control (DMSO; n=5 mice), CPT-11 (5 mg/kg, n=5 mice),
9-ING-41 (70 mg/kg, n=5 mice) and CPT-11+9-ING-41 (n=5 mice). (a) Vehicle (DMSO)
or drugs were injected as shown by arrows. Points, mean tumor volume; bars, SE.
(b) The weight of resected tumors was measured. Columns, mean tumor weight;
bars, SE. (c) Representative pictures of tumors from each group of animals. (d)
The percentage of apoptotic cells was determined by TUNEL staining. Columns,
mean apoptotic cells; bars, SE. (e) Representative pictures of TUNEL staining of
SK-N-DZ neuroblastoma xenograft tumors treated as indicated.