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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Anesthesiology. 2018 Sep;129(3):459–476. doi: 10.1097/ALN.0000000000002281

Figure 1. Tadpoles vs. Zebrafish Larvae.

Figure 1

A: A glass container holding 5 pre-limb bud stage Xenopus tadpoles in 100 mL water. The polished glass rod, visible in the lower right quadrant of the photograph, is used to manually turn the animals during loss-of-righting reflexes tests.

B: A 96-well plate loaded with 64 zebrafish larvae, 1 larva per well in 0.2 mL E3 buffer each. The inset shows a magnified view of one well containing a 7 dpf larva.